HSCRP ELISA | CE CERTIFIED FOR IVD

BRAND:                 DEMEDITEC DIAGNOSTICS GMBH

PRODUCT CODE: DE740011

AVAILABILITY:     IN STOCK

DESCRIPTION

CE certified for IVD use | Precoated plates | Ready to use reagents

• 96 well precoated ELISA microplate

• Incubation time: 1 h 10 min

• Range: 0.4 - 10 µg/ml

• Sensitivity: 0.02 µg/ml

• Sample size: 100 µl (1/1000 predilution)

• Sample type: serum or plasma

• Substrate: TMB 450nm

1. INTENDED USE

High sensitivity immunoenzymetric assay for the in vitro high sensitivity quantitative measurement of human C-reactive protein (hsCRP) in serum and plasma.

2. CLINICAL BACKGROUND

A series of prospective studies have provided consistent data documenting that mild elevation of baseline levels of CRP among apparently healthy individuals is associated with higher long-term risk for future cardiovascular events. The predictive capacity of CRP is independent of traditional cardiovascular risk factors and offers a prognostic advantage over measurement of lipid alone. Inflammatory markers specifically hsCRP may help to identify those who would benefit most from these pharmacological interventions. hsCRP is a novel and evolving biomarker which provides a most useful predictive indicator for subsequent cardiovascular events. This test should not be used for assessment of acute inflammation but should be ordered to evaluate CVD (Cardiovascular Disease) risk in apparently healthy individuals who have not had recent infection or other serious illness.

3. PRINCIPLES OF THE METHOD

Microtiterstrips pre-coated with anti-CRP antibody are incubated with diluted standard sera and patient samples. During this incubation step, CRP is bound specifically to the wells. After removal of the unbound serum proteins by a washing procedure, the antigen-antibody complex in each well is detected with specific peroxidase-conjugated antibodies. After removal of the unbound conjugate, the strips are incubated with a chromogen solution containing tetramethylbenzidine and hydrogen peroxide: a blue colour develops in proportion to the amount of immunocomplex bound to the wells of the strips. The enzymatic reaction is stopped by the addition of 0.5M H2SO4 and the absorbance values at 450 nm are determined. A standard curve is obtained by plotting the absorbance values versus the corresponding standard values. The concentration of CRP in patient samples is determined by interpolation from the standard curve.